Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Artemisia anomala extracts enhance the viability and anti-oxidation capacity of human keratinocytes

Ying Gao, Jing Yuan, Xiaofang Liang, Yimin He, Peng Li, Ming Yang

Department of Dermatology, The Central hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, No.26 Shengli Street, Jingâ€™an distract, Wuhan, Hubei 430014A292;PR China;

For correspondence:- Ming Yang Email: Yangmingiuy@163.com

Accepted: 22 December 2018 Published: 31 January 2019

Citation: Gao Y, Yuan J, Liang X, He Y, Li P, Yang M. Artemisia anomala extracts enhance the viability and anti-oxidation capacity of human keratinocytes. Trop J Pharm Res 2019; 18(1):61-67 doi: 10.4314/tjpr.v18i1.10

© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of extracts of Artemisia anomala S. Moore tissues on viability, apoptosis and antioxidant capacity of human keratinocytes.

Methods: Human keratinocyte cell line HaCaT were treated with extracts of A. anomala for 12 h or 24 h. Cell viability, level of reactive oxygen species (ROS), and incidence of apoptosis were measured by flow cytometry. Levels of mRNA and key proteins in the mitogen-activated protein kinase (MAPK) pathway were determined by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Key proteins of caspase pathways were assessed by western blot. The influence of the extract on the MAPK pathway was further probed by treating cells with MAPK activator in the presence and absence of the extract.

Results: Treatment of cells with extracts of A. anomala enhanced viability and reduced apoptosis in a time-dependent manner, and increased ROS level, compared with control. mRNA and protein expressions of c-Jun N-terminal kinase (JNK), extracellular regulated protein kinase (ERK), and p38 MAPK decreased in extract-treated cells. The extracts also reversed the inhibitory effects of the MAPK pathway activator, actinomycin, on cell viability and ROS, and inhibited protein-cleaved caspase-8 and cleaved caspase-3.

Conclusion: A. anomala extract increases cell viability and antioxidant capacity via inactivation of MAPK pathway, and also inhibits cell apoptosis via inactivation of caspase pathways. Hence, the extract may serve as a promising drug for the treatment of psoriasis.

Keywords: Artemisia anomala, MAPK pathway, Anti-oxidation, Keratinocyte, Psoriasis